The TXNRD2 Polyclonal Antibody (CAB5490) is a valuable tool for research involving thioredoxin reductase 2 (TXNRD2), an essential enzyme involved in redox regulation and antioxidant defense. This antibody, raised in rabbits, exhibits high specificity and sensitivity towards human TXNRD2 samples, making it well-suited for use in Western blot applications. By binding to TXNRD2, this antibody allows for the detection and analysis of the protein in various cell types, facilitating studies in oxidative stress, cancer research, and other fields related to redox biology.TXNRD2 plays a crucial role in maintaining cellular redox balance by catalyzing the reduction of thioredoxin, a key player in antioxidant defense mechanisms.
Dysregulation of TXNRD2 activity has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions. Research into the function and regulation of TXNRD2 is essential for understanding its role in disease pathogenesis and identifying potential therapeutic targets for intervention.Overall, the TXNRD2 Polyclonal Antibody (CAB5490) serves as a valuable tool for investigating the biology of TXNRD2 and its implications in health and disease. Its high specificity and reactivity make it an ideal choice for researchers seeking to delve deeper into the intricate mechanisms of redox signaling and oxidative stress.
Recombinant fusion protein containing a sequence corresponding to amino acids 100-310 of human Thioredoxin reductase 2 (Thioredoxin reductase 2 (TXNRD2 ) ) (NP_006431.2).
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10587
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Thioredoxin reductase 2 (TXNRD2 ) Rabbit pAb (CAB5490) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
