The TXNRD1 Polyclonal Antibody (CAB16631) is a powerful tool for researchers studying TXNRD1, an important enzyme that plays a key role in redox signaling and antioxidant defense mechanisms. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for use in Western blot applications. By targeting the TXNRD1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an invaluable tool for studies in areas such as oxidative stress, cancer biology, and neurodegenerative diseases. TXNRD1, also known as thioredoxin reductase 1, is a crucial enzyme involved in maintaining proper redox balance within cells and preventing oxidative damage.
Dysregulation of TXNRD1 has been linked to various diseases, highlighting its importance as a potential therapeutic target. By studying the function and regulation of TXNRD1, researchers can gain valuable insights into disease mechanisms and potentially develop novel treatments to combat oxidative stress-related disorders. In conclusion, the TXNRD1 Polyclonal Antibody (CAB16631) is a reliable tool for researchers seeking to explore the role of TXNRD1 in cellular function and disease processes. Its high specificity and sensitivity make it well-suited for a variety of experimental techniques, providing valuable data for advancing our understanding of redox biology and its implications for human health.
cytosol, extracellular exosome, fibrillar center, mitochondrion, nucleoplasm
Calculated MW:
71kDa
Observed MW:
65kDa
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis.
Purification Method:
Affinity purification
Gene ID:
7296
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Thioredoxin reductase 1 (TXNRD1 ) Rabbit pAb (CAB16631) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
