The TXN Polyclonal Antibody (CAB0053) is a valuable tool for researchers studying thioredoxin (TXN), a key protein involved in redox regulation and cellular defense against oxidative stress. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.Thioredoxin, a small redox-regulating protein, plays a crucial role in maintaining cellular redox balance and protecting cells from oxidative damage. Its activity is essential for various cellular processes, including DNA repair, protein folding, and cell signaling. Dysregulation of thioredoxin has been implicated in a variety of diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
By targeting the TXN protein, researchers can study the regulation and function of thioredoxin in different cell types and disease states. This antibody enables the detection and analysis of TXN protein levels, allowing for a better understanding of its role in cellular processes and disease development. Its high reactivity and specificity make it a valuable tool for investigations in the fields of molecular biology, biochemistry, and cancer research.
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7295
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Thioredoxin 1 (Trx1/TXN) antibody (CAB0053) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
