The TXA2 (Thromboxane A2) ELISA Kit is specifically designed for the accurate and precise detection of Thromboxane A2 levels in various biological samples such as serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit offers reliable and reproducible results for researchers in the field of inflammation, thrombosis, and cardiovascular diseases.Thromboxane A2 is a key mediator in platelet aggregation and vasoconstriction, playing a critical role in various physiological and pathological processes.
Dysregulation of TXA2 levels has been implicated in conditions such as atherosclerosis, stroke, and myocardial infarction, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the TXA2 ELISA Kit provides researchers with a powerful tool to study the role of Thromboxane A2 in health and disease, offering a comprehensive solution for quantitative analysis in both basic research and clinical applications.
Product Name:
TXA2 (Thromboxane A2) ELISA Kit (UNES00037)
SKU:
UNES00037
Target:
TXA2 (Thromboxane A2) (UNES00037)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with TXA2. During the reaction, TXA2 in the sample or standard competes with a fixed amount of TXA2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to TXA2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TXA2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
97-110
92-109
Average (%)
94
104
99
1:4
Range (%)
84-98
86-101
98-113
Average (%)
89
92
105
1:8
Range (%)
83-97
92-104
99-114
Average (%)
90
99
105
1:16
Range (%)
84-98
89-105
97-109
Average (%)
90
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.9
134.7
401.0
49.1
147.1
433.5
Standard deviation
2.6
7.5
15.6
3.0
7.8
16.9
C V (%)
5.11
5.57
3.89
6.11
5.3
3.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of TXA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes TXA2 in samples. No significant cross-reactivity or interference between TXA2 and analogues was observed.
