The Twist1 Polyclonal Antibody (CAB3237) is a valuable tool for researchers studying Twist1, a transcription factor involved in cancer progression and metastasis. This antibody, generated in rabbits, exhibits high specificity for Twist1 in human samples and is suitable for use in Western blotting applications. By binding to Twist1 protein, it allows for the detection and analysis of Twist1 expression in various cell types, making it ideal for investigations in oncology and cell biology.Twist1 is a key regulator of epithelial-mesenchymal transition (EMT), a process that enables cancer cells to acquire invasive and metastatic properties.
Its role in promoting tumor progression and therapy resistance has made it a target of interest in cancer research. Understanding the mechanisms by which Twist1 influences cancer metastasis is essential for developing new therapeutic strategies to combat advanced and aggressive forms of cancer.Overall, the Twist1 Polyclonal Antibody (CAB3237) provides researchers with a reliable tool for examining Twist1 expression and function in cancer cells, offering insights that may lead to the development of novel treatment approaches for combating metastatic disease.
Product Name:
Twist Rabbit Polyclonal Antibody
SKU:
CAB3237
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of Twist (NP_000465.1).
This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism.
Purification Method:
Affinity purification
Gene ID:
7291
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Twist Rabbit pAb (CAB3237) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
