Anti-TWF2 Antibody (CAB13642)
- SKU:
- CAB13642
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Anti-TWF2 Antibody (CAB13642)
The TWF2 Polyclonal Antibody (CAB13642) is an essential tool for researchers studying TWF2, a protein involved in actin cytoskeleton organization and cell migration. Generated in rabbits, this antibody exhibits high specificity and reactivity towards human samples, making it ideal for Western blot applications. By targeting TWF2, researchers can better understand its role in cellular processes and potentially develop new strategies for treating conditions related to abnormal cell motility and cytoskeletal dynamics.
TWF2, also known as Twinfilin-2, is a key player in regulating actin dynamics, essential for cell movement and shape changes. Dysregulation of TWF2 has been linked to various diseases, including cancer metastasis, cardiovascular disorders, and neurological conditions. Investigating the function of TWF2 with the help of this antibody can provide valuable insights into the mechanisms underlying these pathologies and open up new avenues for therapeutic interventions targeting actin dynamics.
| Antibody Name: | Anti-TWF2 Antibody |
| Antibody SKU: | CAB13642 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat, 293T, A-431, Mouse heart, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAHQ TGIH ATEE LKEF FAKA RAGS VRLI KVVI EDEQ LVLG ASQE PVGR WDQD YDRA VLPL LDAQ QPCY LLYR LDSQ NAQG FEWL FLAW SPDN SPVR LKML YAAT RATV KKEF GGGH IKDE LFGT VKDD LSFA GYQK HLSS CAAP APLT SAER ELQQ IRIN EVKT EISV ESKH QTLQ GLAF PLQP EAQR ALQQ LKQK MVNY IQMK LDLE RETI ELVH TEPT DVAQ LPSR VPRD AARY HFFL YKHT HEGD PLES VVFI YSMP GYKC SIKE RMLY SSCK SRLL DSVE QDFH LEIA KKIE IGDG AELT AEFL YDEV HPKQ HAFK QAFA KPKG PGGK RGHK RLIR GPGE NGDD S |
| Gene ID: | 11344 |
| Uniprot: | Q6IBS0 |
| Cellular Location: | Cell projection, Cytoplasm, cytoskeleton, perinuclear region, stereocilium |
| Calculated MW: | 39kDa |
| Observed MW: | 40kDa |
| Synonyms: | TWF2, A6RP, A6r, MSTP011, PTK9L |
| Background: | The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein. |
| UniProt Protein Function: | A6r: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: stereocilium; cytoskeleton; growth cone; myofibril; lamellipodium; perinuclear region of cytoplasm; cytoplasm; filopodium Molecular Function:actin monomer binding; phosphatidylinositol-4,5-bisphosphate binding; protein kinase C binding; ATP binding Biological Process: cell projection organization and biogenesis; sequestering of actin monomers; negative regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; positive regulation of axon extension; regulation of microvillus length; barbed-end actin filament capping |
| NCBI Summary: | The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6IBS0 |
| NCBI GenInfo Identifier: | 94730596 |
| NCBI Gene ID: | 11344 |
| NCBI Accession: | Q6IBS0.2 |
| UniProt Secondary Accession: | Q6IBS0,Q9Y3F5, |
| UniProt Related Accession: | Q6IBS0 |
| Molecular Weight: | 349 |
| NCBI Full Name: | Twinfilin-2 |
| NCBI Synonym Full Names: | twinfilin actin-binding protein 2 |
| NCBI Official Symbol: | TWF2Â Â |
| NCBI Official Synonym Symbols: | A6r; A6RP; PTK9L; MSTP011Â Â |
| NCBI Protein Information: | twinfilin-2; A6-related protein; twinfilin-1-like protein; twinfilin, actin-binding protein, homolog 2; protein tyrosine kinase 9-like (A6-related protein); PTK9L protein tyrosine kinase 9-like (A6-related protein) |
| UniProt Protein Name: | Twinfilin-2 |
| UniProt Synonym Protein Names: | A6-related protein; hA6RP; Protein tyrosine kinase 9-like; Twinfilin-1-like protein |
| Protein Family: | Twinfilin |
| UniProt Gene Name: | TWF2Â Â |
| UniProt Entry Name: | TWF2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TWF2 antibody (CAB13642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug TWF2 antibody (CAB13642). Western blot was performed from the immunoprecipitate using TWF2 antibody (CAB13642) at a dilition of 1:1000. |
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