The TUBB4A Polyclonal Antibody (PAC046306) is a valuable tool for researchers studying TUBB4A, a key protein involved in neuronal development and function. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.TUBB4A, also known as tubulin beta-4A, plays a crucial role in microtubule dynamics and stability, essential for processes such as cell division, intracellular transport, and neuronal migration. Dysregulation of TUBB4A has been implicated in neurodevelopmental disorders and neurodegenerative diseases, making it a promising target for therapeutic intervention.
By binding specifically to TUBB4A, this antibody enables precise detection and analysis of the protein in various cell types, making it an ideal tool for neurobiology research. Understanding the functions and regulation of TUBB4A is essential for unraveling its contribution to neuronal health and disease, paving the way for potential treatments in neurological disorders.
Western blot. All lanes: TUBB4A antibody at 2 µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 50 kDa. Observed band size: 50, 58 kDa..
Immunofluorescent analysis of MCF-7 cells using PACO46306 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human ovarian cancer using PACO46306 at dilution of 1:100.
Background:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the β chain and one at a non-exchangeable site on the α chain.
TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family.Protein type: Motility/polarity/chemotaxis; CytoskeletalChromosomal Location of Human Ortholog: 16q24.3Cellular Component: microtubule; cell soma; axon; cytoplasm; dendrite; nucleusMolecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; peptide bindingBiological Process: protein polymerization; axon guidance; mitosis; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based processDisease: Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement; Cortical Dysplasia, Complex, With Other Brain Malformations 1
UniProt Protein Details:
NCBI Summary:
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
