The TTLL6 Polyclonal Antibody (PAC037950) is a valuable tool for researchers studying the TTLL6 protein, a member of the tubulin tyrosine ligase-like protein family implicated in various cellular processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically targeting the TTLL6 protein, this antibody allows for the detection and analysis of TTLL6 expression in different cell types, making it ideal for investigations in cell biology and developmental biology.TTLL6 is known to play a key role in the post-translational modification of tubulin through the addition of tyrosine ligands, which is essential for microtubule function and stability.
Dysfunction of TTLL6 has been associated with defects in cilia formation and neuronal development, highlighting its importance in cellular processes. Research on TTLL6 is crucial for understanding its role in health and disease, particularly in disorders related to cilia dysfunction and neurodevelopmental abnormalities. By studying the function of TTLL6, researchers can gain valuable insights into potential therapeutic targets for these conditions.
Western Blot. Positive WB detected in: U87 whole cell lysate. All lanes: TTLL6 antibody at 5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 97, 65 kDa. Observed band size: 97 kDa.
IHC image of PACO37950 diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Polyglutamylase which preferentially modifies alpha-tubulin. Mediates tubulin polyglutamylation in cilia. Involved in the side-chain elongation step of the polyglutamylation reaction rather than in the initiation step.
Polyglutamylase which preferentially modifies alpha-tubulin. Mediates tubulin polyglutamylation in cilia. Involved in the side-chain elongation step of the polyglutamylation reaction rather than in the initiation step. Generates long side-chains. Generates polyglutamylation of CGAS, leading to impair the DNA-binding activity of CGAS.
