The TSG101/VPS23 Monoclonal Antibody is a valuable tool for researchers studying the TSG101 and VPS23 proteins, which are key players in the endosomal sorting complex required for transport (ESCRT) pathway. This monoclonal antibody, produced using hybridoma technology, exhibits high specificity and sensitivity for detecting TSG101 and VPS23 in various experimental applications, including immunofluorescence, immunoprecipitation, and flow cytometry.The ESCRT pathway is essential for sorting ubiquitinated membrane proteins into multivesicular bodies for degradation, as well as for virus budding and cytokinesis. Dysregulation of the ESCRT machinery has been implicated in various diseases, including cancer, neurodegenerative disorders, and viral infections.
By targeting TSG101 and VPS23, researchers can gain insights into the molecular mechanisms underlying these pathologies and develop new therapeutic strategies.Overall, the TSG101/VPS23 Monoclonal Antibody is a valuable tool for investigating the ESCRT pathway and its role in cellular processes and disease mechanisms. Its specificity, sensitivity, and versatility make it an essential reagent for a range of research applications in cell biology, virology, and therapeutic development.
Product Name:
TSG101/VPS23 Monoclonal Antibody
SKU:
CAB22762
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthesized peptide derived from human TSG101/VPS23.
Tested Applications:
IHC-PELISA
Recommended Dilution:
IHC-P,1:50 - 1:200
Synonyms:
TSG10; VPS23; TSG101/VPS23
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Late endosome membrane, Membrane, Nucleus, Peripheral membrane protein.
Calculated MW:
44kDa
Observed MW:
Refer to figures
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
Purification Method:
Affinity purification
Gene ID:
7251
Clone Number:
ARC57589
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded mouse brain using TSG101/VPS23 Rabbit mAb (CAB22762) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
