The TSG101 Polyclonal Antibody (CAB1692) is a powerful tool for researchers studying TSG101, a key protein involved in cellular trafficking and signaling pathways. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically targeting the TSG101 protein, this antibody allows for accurate detection and analysis in various cell types, making it an essential component for studies in cell biology and cancer research.
TSG101 plays a vital role in intracellular protein sorting and transport, as well as in regulating cellular signaling cascades. Its involvement in these critical processes makes it a target of interest for investigating diseases such as cancer and viral infections. By understanding the function of TSG101, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.
Product Name:
TSG101/VPS23 Polyclonal Antibody
SKU:
CAB1692
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 291-390 of human TSG101/VPS23 (NP_006283.1).
Cytoplasm, Late endosome membrane, Membrane, Nucleus, Peripheral membrane protein.
Calculated MW:
44kDa
Observed MW:
44kDa
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
Purification Method:
Affinity purification
Gene ID:
7251
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and TSG101/VPS23 knockdown (KD) SH-SY5Y cells using TSG101/VPS23 Rabbit pAb (CAB1692) at 1:500 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 120s.
