Anti-TRPM2 Antibody (CAB6137)

Antibody Name:	Anti-TRPM2 Antibody
Antibody SKU:	CAB6137
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human TRPM2 (NP_003298.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Mouse, Rat
Positive Samples:	Mouse lung

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human TRPM2 (NP_003298.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLDF ILFC LRLM HIFT ISKT LGPK IIIV KRMM KDVF FFLF LLAV WVVS FGVA KQAI LIHN ERRV DWLF RGAV YHSY LTIF GQIP GYID GVNF NPEH CSPN G
Gene ID:	7226
Uniprot:	O94759
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	95kDa/165kDa/171kDa
Observed MW:	171KDa

Synonyms:

TRPM2, EREG1, KNP3, LTRPC2, LTrpC-2, NUDT9H, NUDT9L1, TRPC7

Background:

The protein encoded by this gene forms a tetrameric cation channel that is permeable to calcium, sodium, and potassium and is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. Additional transcript variants of this gene have been described, but their full-length nature is not known.

UniProt Protein Function:	TRPM2: Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transporter; EC 3.6.1.13; Membrane protein, integral; Channel, calcium; Membrane protein, multi-pass; Hydrolase Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:ADP-ribose diphosphatase activity; calcium channel activity; manganese ion transmembrane transporter activity; sodium channel activity Biological Process: response to hydroperoxide; metabolic process; calcium ion transport; transmembrane transport
NCBI Summary:	The protein encoded by this gene forms a tetrameric cation channel that is permeable to calcium, sodium, and potassium and is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. Additional transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2016]
UniProt Code:	O94759
NCBI GenInfo Identifier:	160381681
NCBI Gene ID:	7226
NCBI Accession:	O94759.2
UniProt Related Accession:	O94759
Molecular Weight:	~ 171kDa
NCBI Full Name:	Transient receptor potential cation channel subfamily M member 2
NCBI Synonym Full Names:	transient receptor potential cation channel subfamily M member 2
NCBI Official Symbol:	TRPM2
NCBI Official Synonym Symbols:	KNP3; EREG1; TRPC7; LTRPC2; NUDT9H; LTrpC-2; NUDT9L1
NCBI Protein Information:	transient receptor potential cation channel subfamily M member 2
UniProt Protein Name:	Transient receptor potential cation channel subfamily M member 2
UniProt Synonym Protein Names:	Estrogen-responsive element-associated gene 1 protein; Long transient receptor potential channel 2; LTrpC-2; LTrpC2; Transient receptor potential channel 7; TrpC7
Protein Family:	Transient receptor potential cation channel subfamily
UniProt Gene Name:	TRPM2
UniProt Entry Name:	TRPM2_HUMAN

	Western blot analysis of extracts of Mouse lung, using TRPM2 antibody (CAB6137) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00062). Exposure time: 90s.
	Immunofluorescence analysis of rat brain using TRPM2 antibody (CAB6137) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse brain using TRPM2 antibody (CAB6137) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)