The TRPC4AP Antibody (PAC0173-25) is a high-quality polyclonal antibody specifically designed for research involving TRPC4AP, a protein involved in calcium signaling and cell proliferation. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the TRPC4AP protein, researchers can accurately detect and analyze its expression in a variety of cell types, making it an ideal tool for studies in cell biology and signal transduction pathways.TRPC4AP is a crucial component in calcium signaling pathways, playing a key role in regulating cell growth and proliferation.
Understanding the function of TRPC4AP is essential for research into various diseases, including cancer and neurological disorders, where aberrant calcium signaling can contribute to disease pathology. By targeting TRPC4AP, researchers can gain valuable insights into the molecular mechanisms underlying these diseases and potentially develop new therapeutic strategies to target this protein for treatment.
Antibody Name:
TRPC4AP Antibody (PACO17325)
Antibody SKU:
PACO17325
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:30-1:150
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human TRPC4AP
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO17325(TRPC4AP Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200).
Background:
TRPC4AP (transient receptor potential cation channel, subfamily C, member 4 associated protein), also known as TRUSS or TRRP4AP, is a 797 amino acid, protein that is expressed in a variety of tissues, with highest expression in liver, heart, testis and brain. Thought to function as a scaffolding protein, TRPC4AP interacts with TNF-R1 and may both link TNF-R1 to the IKK signalsome complex, and participate in the activation of NF κ B p50, an event that occurs in response to TNF-R1 ligation. TRPC4AP exists as multiple alternatively spliced isoforms that are encoded by a gene which maps to human chromosome 20. Comprising approximately 2% of the human genome, chromosome 20 contains nearly 63 million bases that encode over 600 genes, some of which are associated with Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome.
Synonyms:
transient receptor potential cation channel, subfamily C, member 4 associated protein
UniProt Protein Function:
TRPC4AP: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca(2+) storage reduction in response to CHRM1. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Adaptor/scaffoldChromosomal Location of Human Ortholog: 20q11.22Cellular Component: plasma membraneMolecular Function: calcium channel activity; phosphatase binding; protein bindingBiological Process: protein ubiquitination; ubiquitin-dependent protein catabolic process
