The TRMT1 Polyclonal Antibody (CAB7116) is specifically designed for researchers studying TRMT1, an enzyme involved in tRNA modification. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is ideal for use in Western blot applications. TRMT1 plays a crucial role in maintaining the stability and functionality of tRNAs, essential for accurate protein synthesis in cells.
By targeting the TRMT1 protein, this antibody enables the detection and analysis of TRMT1 in various cell types, making it a valuable tool for studies in molecular biology and RNA processing. Understanding the function of TRMT1 is vital for unraveling the mechanisms of protein translation and uncovering potential therapeutic targets in diseases related to tRNA dysregulation.
Product Name:
TRMT1 Rabbit Polyclonal Antibody
SKU:
CAB7116
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 460-659 of human TRMT1 (NP_060192.1).
This gene encodes a tRNA-modifying enzyme that acts as a dimethyltransferase, modifying a single guanine residue at position 26 of the tRNA. The encoded enzyme has both mono- and dimethylase activity when exogenously expressed, and uses S-adenosyl methionine as a methyl donor. The C-terminal region of the encoded protein has both a zinc finger motif, and an arginine/proline-rich region. Mutations in this gene have been implicated in autosomal recessive intellectual disorder (ARID). Alternative splicing results in multiple transcript variants encoding different isoforms. There is a pseudogene of this gene on the X chromosome.
Purification Method:
Affinity purification
Gene ID:
55621
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using TRMT1 Rabbit pAb (CAB7116) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
