The Trimethyl Histone H4 (K20) Polyclonal Antibody (CAB2372) is a valuable tool for researchers studying histone modifications and epigenetics. This antibody, generated in rabbits, is highly specific to the trimethylated form of histone H4 at lysine 20 and is suitable for use in a variety of applications, including Western blot and immunohistochemistry.Histone H4 lysine 20 trimethylation is a crucial epigenetic mark associated with gene repression and chromatin compaction. Dysregulation of this modification has been linked to various diseases, including cancer and developmental disorders. The CAB2372 antibody enables precise detection and analysis of trimethyl histone H4 (K20) levels in different cell types and tissues, making it an essential tool for epigenetic research.
Understanding the role of histone modifications, such as H4 lysine 20 trimethylation, is key to unraveling the complexity of gene regulation and chromatin dynamics. The CAB2372 antibody provides researchers with a reliable means to investigate the impact of this specific histone modification on gene expression and cellular processes, paving the way for new insights into epigenetic mechanisms and potential therapeutic targets.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TriMethyl-Histone H4-K20 Rabbit pAb (CAB2372) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
