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Anti-TriMethyl-Histone H3-K9 Antibody (CAB2360)

SKU:
CAB2360
Product Type:
Antibody
Applications:
WB
IHC
IF
IP
ChIP
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-TriMethyl-Histone H3-K9 Antibody CAB2360
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-TriMethyl-Histone H3-K9 Antibody CAB2360
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-TriMethyl-Histone H3-K9 Antibody CAB2360
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-TriMethyl-Histone H3-K9 Antibody CAB2360
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-TriMethyl-Histone H3-K9 Antibody CAB2360
$167 - $503
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Description

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Anti-TriMethyl-Histone H3-K9 Antibody (CAB2360)

The Trimethyl-Histone H3 (K9) Polyclonal Antibody (CAB2360) is a valuable tool for researchers studying epigenetics and chromatin remodeling. This antibody, produced in rabbits, is highly specific to the trimethylated form of Histone H3 at lysine 9 (H3K9me3), a histone modification associated with gene silencing and heterochromatin formation.Histone modifications play a critical role in regulating gene expression, and H3K9me3 is particularly important in maintaining genome stability and controlling cell fate. The CAB2360 antibody enables the detection and analysis of H3K9me3 levels in various cell types and tissues, making it ideal for studies in cancer research, developmental biology, and epigenetics.

By targeting the trimethylated form of Histone H3 at lysine 9, researchers can investigate the role of this epigenetic mark in gene regulation, cell differentiation, and disease progression. Understanding the mechanisms underlying H3K9me3 deposition and removal is essential for developing novel therapeutic strategies targeting epigenetic dysregulation in cancer and other diseases.

Antibody Name:Anti-TriMethyl-Histone H3-K9 Antibody
Antibody SKU:CAB2360
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP ChIP ChIPseq
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Host Species:Rabbit
Immunogen:A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Application:WB IHC IF IP ChIP ChIPseq
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:50 - 1:200 ChIPseq 1:50 - 1:200
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Positive Samples:HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein
Immunogen:A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8290
Uniprot:Q16695
Cellular Location:Chromosome, Nucleus
Calculated MW:15kDa
Observed MW:17KDa
Synonyms:H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
UniProt Protein Function:HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
UniProt Code:Q16695
NCBI GenInfo Identifier:18202512
NCBI Gene ID:8290
NCBI Accession:Q16695.3
UniProt Secondary Accession:Q16695,Q6FGU4, B2R5K3,
UniProt Related Accession:Q16695
Molecular Weight:
NCBI Full Name:Histone H3.1t
NCBI Synonym Full Names:histone cluster 3, H3
NCBI Official Symbol:HIST3H3  
NCBI Official Synonym Symbols:H3t; H3.4; H3/g; H3FT  
NCBI Protein Information:histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name:Histone H3.1t
UniProt Synonym Protein Names:H3/g
UniProt Gene Name:HIST3H3  
UniProt Entry Name:H31T_HUMAN
Western blot - TriMethyl-Histone H3-K9 Rabbit pAb Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
- TriMethyl-Histone H3-K9 Rabbit pAb Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody (A2360).
Immunohistochemistry - TriMethyl-Histone H3-K9 Rabbit pAb Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Immunohistochemistry - TriMethyl-Histone H3-K9 Rabbit pAb Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Immunofluorescence - TriMethyl-Histone H3-K9 Rabbit pAb Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K9 antibody (A2360). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation - TriMethyl-Histone H3-K9 Rabbit pAb Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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