The Trimethyl Histone H3 (K79) Polyclonal Antibody (CAB2369) is a crucial tool for researchers studying epigenetic modifications in histone proteins. This antibody, generated in rabbits, specifically targets the trimethylated form of histone H3 at lysine 79, a modification associated with transcriptional regulation and DNA repair processes.Histone modifications play a key role in regulating gene expression by altering the chromatin structure, thereby influencing DNA accessibility for transcription factors. The trimethylation of histone H3 at lysine 79 is linked to various cellular processes, including DNA damage response and cell cycle regulation.With its high specificity and sensitivity, the Trimethyl Histone H3 (K79) Polyclonal Antibody enables researchers to detect and analyze this specific histone modification in various cell types and experimental conditions.
Its validation for use in Western blot applications ensures reliable results, making it an ideal choice for studies in epigenetics, cancer research, and developmental biology.By investigating the role of trimethylated histone H3 at lysine 79, researchers can gain valuable insights into the mechanisms underlying gene regulation and cellular function, paving the way for potential therapeutic interventions targeting epigenetic pathways in disease.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using TriMethyl-Histone H3-K79 Rabbit pAb (CAB2369) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 300s.
