The Trimethyl-Histone H3 (K64) Polyclonal Antibody (CAB7259) is specifically designed for research involving histone H3, a crucial protein involved in chromatin structure and gene regulation. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications such as Western blot and immunohistochemistry.Histone H3 trimethylation at lysine 64 (H3K64me3) is an epigenetic modification that plays a role in gene expression regulation and chromatin organization. Aberrant levels of H3K64me3 have been implicated in various diseases, including cancer and neurological disorders.
This antibody specifically targets the trimethylated form of histone H3 at lysine 64, allowing for precise detection and analysis of this epigenetic mark in different cell types.The Trimethyl-Histone H3 (K64) Polyclonal Antibody is a valuable tool for researchers studying epigenetics, chromatin biology, and diseases linked to histone modifications. By understanding the role of H3K64me3 in gene regulation and disease development, this antibody can provide important insights into potential therapeutic targets and treatment strategies.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using TriMethyl-Histone H3-K64 Rabbit pAb (CAB7259) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 150s.
