The Trimethyl Histone H3 (K56) Polyclonal Antibody (CAB7262) is a valuable tool for researchers studying epigenetic modifications related to histone H3. This antibody, produced in rabbits, is highly specific to the trimethylated form of histone H3 at lysine 56 (H3K56me3) and has been validated for use in various applications, including Western blot and immunohistochemistry.Histone H3 is a key component of chromatin structure and plays a crucial role in gene expression regulation. The trimethylation of H3K56 is associated with transcriptional activation and DNA damage response pathways. By targeting this specific histone modification, researchers can investigate the role of H3K56me3 in various cellular processes and diseases, such as cancer, neurodegenerative disorders, and developmental abnormalities.
With its high reactivity and specificity for H3K56me3, the Trimethyl Histone H3 (K56) Polyclonal Antibody is a valuable tool for scientists conducting research in epigenetics, chromatin biology, and genetic regulation. Its ability to detect and analyze this specific histone modification in different cell types makes it an essential resource for studies aiming to unravel the complex mechanisms underlying gene regulation and disease development.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using TriMethyl-Histone H3-K56 Rabbit pAb (CAB7262) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
