The Trimethyl Histone H3 (K36) Polyclonal Antibody (CAB2366) is a valuable tool for researchers studying histone modifications and their role in gene expression regulation. This antibody, raised in rabbits, specifically targets trimethylated histone H3 at lysine 36 and is highly reactive with human samples. Validated for use in techniques such as Western blotting, this antibody allows for the detection and analysis of trimethylated histone H3 (K36) in a variety of cell types.Histone modifications, such as trimethylation of histone H3 at lysine 36, play a crucial role in epigenetic regulation of gene expression.
Dysregulation of these modifications has been implicated in various diseases, including cancer, making the Trimethyl Histone H3 (K36) Polyclonal Antibody a valuable tool for researchers studying the molecular mechanisms underlying these conditions. By understanding the role of trimethylated histone H3 (K36) in gene regulation, scientists can gain insight into disease pathogenesis and potentially identify new therapeutic targets for intervention.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TriMethyl-Histone H3-K36 Rabbit pAb (CAB2366) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
