null

TRIM8 Antibody (PACO57480)

SKU:
PACO57480
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
IHC
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • TRIM8 Antibody PACO57480
  • TRIM8 Antibody PACO57480
€359

Description

system_update_altDatasheetsystem_update_altMSDS

TRIM8 Antibody (PACO57480)

The TRIM8 Polyclonal Antibody (PACO57480) is a valuable tool for researchers studying the TRIM8 protein, known for its role in immune regulation and anti-viral responses. This antibody is produced in rabbits and shows high reactivity with human samples, making it an ideal choice for Western blot applications. By targeting the TRIM8 protein, this antibody enables precise detection and analysis in a variety of cell types, providing valuable insights for immunology and virology research.TRIM8, a member of the TRIM protein family, has been implicated in various cellular processes, including immune signaling and antiviral defense mechanisms.

Its involvement in these pathways makes it a key target for investigating diseases related to immune dysfunction and viral infections. By using the TRIM8 Polyclonal Antibody, researchers can gain a better understanding of the molecular mechanisms underlying these conditions and potentially identify new therapeutic strategies for combating them.

Antibody Name:TRIM8 Antibody (PACO57480)
Antibody SKU:PACO57480
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, IHC, IF
Recommended Dilutions:ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:Human
Immunogen:Recombinant Human Probable E3 ubiquitin-protein ligase TRIM8 protein (367-517AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageImmunofluorescence staining of HepG2 cells with PACO57480 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product ImageIHC image of PACO57480 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1.
Synonyms:E3 ubiquitin-protein ligase TRIM8 (EC 2.3.2.27) (Glioblastoma-expressed RING finger protein) (RING finger protein 27) (RING-type E3 ubiquitin transferase TRIM8) (Tripartite motif-containing protein 8), TRIM8, GERP RNF27
UniProt Protein Function:TRIM8: Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1. Belongs to the TRIM/RBCC family.Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligaseChromosomal Location of Human Ortholog: 10q24.32Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm; PML bodyMolecular Function: identical protein binding; protein binding; protein homodimerization activityBiological Process: activation of NF-kappaB transcription factor; innate immune response; positive regulation of autophagy; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transcription factor activity
UniProt Protein Details:
NCBI Summary:This gene encodes a member of the tripartite motif (TRIM) protein family. Based on similarities to other proteins, the encoded protein is suspected to be an E3 ubiquitin-protein ligase. Regulation of this gene may be altered in some cancers. Mutations resulting in a truncated protein product have been observed in early-onset epileptic encephalopathy (EOEE). [provided by RefSeq, Sep 2016]
UniProt Code:Q9BZR9
NCBI GenInfo Identifier:12382258
NCBI Gene ID:81603
NCBI Accession:AAG53087.1
UniProt Secondary Accession:Q9BZR9,Q9C028, A6NI31
UniProt Related Accession:Q9BZR9
Molecular Weight:61,489 Da
NCBI Full Name:glioblastoma-expressed RING finger protein GERP
NCBI Synonym Full Names:tripartite motif containing 8
NCBI Official Symbol:TRIM8  
NCBI Official Synonym Symbols:GERP; RNF27  
NCBI Protein Information:probable E3 ubiquitin-protein ligase TRIM8
UniProt Protein Name:Probable E3 ubiquitin-protein ligase TRIM8
UniProt Synonym Protein Names:Glioblastoma-expressed RING finger protein; RING finger protein 27; RING-type E3 ubiquitin transferase TRIM8Curated; Tripartite motif-containing protein 8
Protein Family:
UniProt Gene Name:TRIM8  
UniProt Entry Name:
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
Recommended Products
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011)
Anti-HRP-conjugated Beta Actin Antibody (CABC028)

Related Products