The TRIM71 Polyclonal Antibody (PACO57164) is a valuable tool for researchers studying TRIM71, a protein involved in various cellular processes including RNA regulation and stem cell differentiation. This antibody, produced in rabbits, demonstrates high specificity and sensitivity for detecting TRIM71 in human samples, making it ideal for use in Western blot and immunofluorescence applications.TRIM71, also known as LIN41, is a key player in post-transcriptional gene regulation and has been implicated in stem cell pluripotency and differentiation. Its dysregulation has been linked to various diseases, including cancer and neurodevelopmental disorders, highlighting the importance of studying its function.
The TRIM71 Polyclonal Antibody enables researchers to investigate the expression and localization of TRIM71 in different cell types, providing valuable insights into its role in disease development and progression.By using the TRIM71 Polyclonal Antibody, researchers can further unravel the complex mechanisms underlying TRIM71-mediated processes and potentially identify new therapeutic targets for a range of diseases. Its high specificity and reliability make it a valuable tool for advancing research in molecular biology, developmental biology, and disease pathology.
Antibody Name:
TRIM71 Antibody (PACO57164)
Antibody SKU:
PACO57164
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:500-1:1000
Species Reactivity:
Human
Immunogen:
Recombinant Human E3 ubiquitin-protein ligase TRIM71 protein (125-209AA)
IHC image of PACO57164 diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO57164 diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1.
TRIM71: May be involved in controlling the timing of organ formation during development. Belongs to the TRIM/RBCC family.Chromosomal Location of Human Ortholog: 3p22.3Molecular Function: miRNA binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activityBiological Process: neural tube development; protein autoubiquitination; fibroblast growth factor receptor signaling pathway; neural tube closure; miRNA-mediated gene silencing, negative regulation of translation; G1/S transition of mitotic cell cycle
