The TRIM47 Antibody (CAB17803) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific to human samples and has been rigorously validated for use in Western blot applications. TRIM47, a member of the tripartite motif-containing (TRIM) protein family, is known for its role in regulating immune signaling pathways and modulating inflammatory responses. Its expression has been linked to conditions such as cancer, autoimmune disorders, and viral infections, making it an important target for research in immunology and disease pathology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRIM47 Antibody
SKU:
CAB17803
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GOA, RNF100, TRIM47
Positive Sample:
Mouse stomach
Cellular Localization:
Cytosol, Nucleus.
Calculated MW:
70kDa
Observed MW:
76kDa
Enables ubiquitin-protein transferase activity. Involved in protein ubiquitination. Located in cytosol.
Purification Method
Affinity purification
Gene ID
91107
RRID
AB_2772713
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse stomach, using TRIM47 Rabbit pAb (CAB17803) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of L929 cells using TRIM47 Rabbit pAb (CAB17803) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
