The TRIM3 Polyclonal Antibody (PAC020358) is a valuable tool for researchers studying TRIM3, a protein involved in various cellular processes including protein degradation, cellular differentiation, and neuronal development. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the TRIM3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it a versatile tool for research in neurobiology, cell biology, and cancer biology.TRIM3, also known as an E3 ubiquitin ligase, plays a crucial role in regulating protein stability and degradation within cells.
Its functions are diverse and can impact processes such as cell proliferation, differentiation, and signaling pathways. Research into TRIM3 is essential for understanding its role in neural development, synaptic plasticity, and neurodegenerative diseases. The TRIM3 Polyclonal Antibody provides researchers with a reliable tool to explore the functions and mechanisms of TRIM3 in various biological contexts, paving the way for new insights into cellular regulation and disease pathogenesis.
Antibody Name:
TRIM3 Antibody (PACO20358)
Antibody SKU:
PACO20358
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide of human TRIM3
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO20358(TRIM3 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO20358(TRIM3 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4.
Synonyms:
tripartite motif containing 3
UniProt Protein Function:
TRIM3: Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Belongs to the TRIM/RBCC family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Cytoskeletal; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: cytoplasm; early endosome
Molecular Function:protein C-terminus binding; protein binding; zinc ion binding; ubiquitin-protein ligase activity
Biological Process: nervous system development; protein transport; protein ubiquitination
NCBI Summary:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]
tripartite motif-containing protein 3; RING finger protein 97; ring finger protein 22; brain expressed ring finger; tripartite motif-containing 3; tripartite motif protein TRIM3; brain-expressed RING finger protein
UniProt Protein Name:
Tripartite motif-containing protein 3
UniProt Synonym Protein Names:
Brain-expressed RING finger protein; RING finger protein 22; RING finger protein 97
