The TRIM3 Polyclonal Antibody (PACO20357) is a valuable tool for researchers studying TRIM3, a protein involved in various cellular processes including protein degradation and cellular trafficking. This antibody is produced in rabbits and has been validated for use in Western blot applications, providing accurate and reliable results. By targeting the TRIM3 protein, this antibody enables the detection and analysis of TRIM3 in different cell types, making it an essential tool for studies in molecular biology and signal transduction pathways.
TRIM3, also known as tripartite motif-containing 3, is a key player in cellular homeostasis and has been implicated in diseases such as cancer and neurodegenerative disorders. By investigating the role of TRIM3 in these conditions, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets. This antibody opens up new possibilities for understanding the functions of TRIM3 and its implications in various physiological and pathological processes.
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO20357(TRIM3 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 6%SDS-PAGE, Lysate: 40 µg, Lane 1-2: Mouse brain tissue, human fetal brain tissue, Primary antibody: PACO20357(TRIM3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20357(TRIM3 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4.
Synonyms:
tripartite motif containing 3
UniProt Protein Function:
TRIM3: Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Belongs to the TRIM/RBCC family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Cytoskeletal; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: cytoplasm; early endosome
Molecular Function:protein C-terminus binding; protein binding; zinc ion binding; ubiquitin-protein ligase activity
Biological Process: nervous system development; protein transport; protein ubiquitination
NCBI Summary:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]
tripartite motif-containing protein 3; RING finger protein 97; ring finger protein 22; brain expressed ring finger; tripartite motif-containing 3; tripartite motif protein TRIM3; brain-expressed RING finger protein
UniProt Protein Name:
Tripartite motif-containing protein 3
UniProt Synonym Protein Names:
Brain-expressed RING finger protein; RING finger protein 22; RING finger protein 97
