The TRIM25 Polyclonal Antibody (CAB12938) is a valuable tool for researchers studying TRIM25, an important protein involved in immune response regulation and antiviral defense mechanisms. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples, making it an ideal choice for Western blotting applications.TRIM25, also known as tripartite motif-containing protein 25, plays a crucial role in the innate immune response by functioning as an E3 ubiquitin ligase and regulating the activation of the RIG-I signaling pathway. By targeting viral RNA and promoting its degradation, TRIM25 contributes to the antiviral defense mechanisms within the host.
The TRIM25 Polyclonal Antibody enables researchers to detect and analyze the expression levels of TRIM25 in various cell types, providing valuable insights into its role in immune regulation and viral defense. This antibody is a valuable tool for studies in virology, immunology, and infectious diseases, and its use can contribute to the development of novel therapeutic strategies targeting TRIM25 in diseases such as viral infections and autoimmune conditions.
Product Name:
TRIM25 Rabbit Polyclonal Antibody
SKU:
CAB12938
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 190-379 of human TRIM25 (NP_005073.2).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
EFP; Z147; RNF147; ZNF147; TRIM25
Positive Sample:
HeLa,293T,MCF7
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
71kDa
Observed MW:
72kDa
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity.
Purification Method:
Affinity purification
Gene ID:
7706
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TRIM25 Rabbit pAb (CAB12938) at 1:1500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
