The Tri-Methyl Histone H2B Lys5 Antibody (PACO00180) is a powerful tool for researchers studying epigenetics and chromatin regulation. This polyclonal antibody, raised in rabbits, is highly specific and sensitive for detecting levels of tri-methylated histone H2B at lysine 5 in human samples. Validated for use in techniques such as Western blot and immunohistochemistry, this antibody allows for precise analysis of histone modification patterns in various cell types.Histone modifications, such as tri-methylation of histone H2B at lysine 5, play a crucial role in gene expression regulation and chromatin structure dynamics. Dysregulation of histone modifications has been implicated in various diseases, including cancer and developmental disorders.
By targeting specific histone marks like tri-methylated H2B Lys5, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the Tri-Methyl Histone H2B Lys5 Antibody is a valuable tool for researchers interested in exploring the intricate world of epigenetics and chromatin biology. Its high specificity and versatility make it an essential component for studies aiming to unravel the complex relationship between histone modifications and gene regulation.
Antibody Name:
Tri-Methyl-Histone H2B (Lys5) Antibody
Antibody SKU:
PACO00180
Size:
50ug
Host Species:
Rabbit
Tested Applications:
WB
Recommended Dilutions:
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthetic Peptide
Form:
Liquid
Storage Buffer:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
H2B1A: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Transcribed exclusively in testis, and the corresponding protein is also present in mature sperm. Belongs to the histone H2B family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p22.2Cellular Component: extrinsic to plasma membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleusMolecular Function: DNA binding; protein heterodimerization activityBiological Process: establishment and/or maintenance of chromatin architecture; inflammatory response; nucleosome assembly; nucleosome disassembly; plasminogen activation; positive regulation of binding; spermatogenesis, exchange of chromosomal proteins
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]
