The Tri-Methyl Histone H1 Lys25 Antibody (PAC00182) is a highly specific antibody designed for research involving histone modification, specifically focusing on the tri-methylation of histone H1 at lysine 25. This modification plays a critical role in gene regulation and chromatin structure, making the antibody a valuable tool for studying epigenetic mechanisms.Raised in rabbits, this polyclonal antibody is highly reactive with human samples and has been validated for use in various applications, including Western blotting. By binding specifically to the tri-methylated histone H1 at lysine 25, researchers can effectively detect and analyze this modification in different cell types, providing insights into its role in gene expression and cellular processes.
The Tri-Methyl Histone H1 Lys25 Antibody is ideal for studies in epigenetics, chromatin biology, and gene regulation. By understanding the impact of histone modifications on gene expression, researchers can unravel the complexities of epigenetic regulation and potentially uncover new therapeutic targets for diseases such as cancer, developmental disorders, and neurological conditions. Overall, this antibody offers a powerful tool for investigating the intricate mechanisms of histone modifications, paving the way for advancements in molecular biology and therapeutic development.
Antibody Name:
Tri-Methyl-Histone H1 (Lys25) Antibody
Antibody SKU:
PACO00182
Size:
50ug
Host Species:
Rabbit
Tested Applications:
WB
Recommended Dilutions:
Species Reactivity:
Human
Immunogen:
synthetic Peptide
Form:
Liquid
Storage Buffer:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
H1F1: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Belongs to the histone H1/H5 family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p21.3Cellular Component: nuclear chromatin; nucleosomeMolecular Function: chromatin DNA binding; protein bindingBiological Process: nucleosome assembly; spermatogenesis
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
