The Trefoil Factor 2 Polyclonal Antibody (CAB5423) is a valuable tool for researchers studying Trefoil Factor 2 (TFF2), a protein involved in mucosal protection and repair. This antibody, produced in rabbits, showcases high reactivity with human samples and has been validated for use in Western blot applications. By binding to the TFF2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in gastroenterology and gastrointestinal research.
TFF2, also known as spasmolytic polypeptide, plays a crucial role in maintaining the integrity of the mucosal barrier in the gastrointestinal tract. Its involvement in promoting mucosal healing and protecting against gastrointestinal damage underscores its significance in understanding conditions such as inflammatory bowel disease, gastritis, and peptic ulcers. Investigating the function of TFF2 is key to developing targeted therapies that support mucosal health and repair in these pathological contexts.
Product Name:
TFF2 Rabbit Polyclonal Antibody
SKU:
CAB5423
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-129 of human TFF2 (NP_005414.1).
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
Purification Method:
Affinity purification
Gene ID:
7032
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HT-29 cells, using TFF2 antibody (CAB5423) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
