The Transketolase Polyclonal Antibody (CAB6314) is a powerful tool for research involving transketolase, an essential enzyme involved in the pentose phosphate pathway. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.Transketolase is a key enzyme in glucose metabolism, playing a crucial role in the production of NADPH and ribose-5-phosphate, important for cell growth and proliferation. Dysregulation of transketolase has been linked to various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a promising target for therapeutic interventions.
By utilizing the Transketolase Polyclonal Antibody, researchers can analyze the expression and activity of transketolase in different cell types and tissue samples, providing valuable insights into metabolic pathways and potential therapeutic strategies. Its specificity and sensitivity make it an essential tool for studies in biochemistry, molecular biology, and disease research.
Product Name:
TKT Rabbit Polyclonal Antibody
SKU:
CAB6314
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TKT (NP_001128527.1).
This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
7086
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TKT Rabbit pAb (CAB6314) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
