The TPX2 Polyclonal Antibody (CAB18327) is a high-quality research tool designed for investigating TPX2, a critical protein involved in cell division and spindle assembly. This rabbit polyclonal antibody is specifically optimized for use in various applications, including Western blot, immunofluorescence, immunoprecipitation, and flow cytometry.TPX2 is a well-known regulator of microtubule organization and plays a crucial role in ensuring proper chromosome segregation during cell division. Dysregulation of TPX2 expression has been linked to cancer development and progression, making it a promising target for cancer research and drug development.
The TPX2 Polyclonal Antibody, validated for use in human samples, provides researchers with a reliable tool for studying the expression and localization of TPX2 in different cell types and tissues. Its high specificity and sensitivity make it ideal for investigating the functions of TPX2 in normal cellular processes and disease states, ultimately contributing to our understanding of cell division and its implications in cancer biology.
Product Name:
TPX2 Rabbit Polyclonal Antibody
SKU:
CAB18327
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TPX2 (NP_036244.2).
Cytoplasm, Nucleus, cytoskeleton, spindle, spindle pole.
Calculated MW:
86kDa
Observed MW:
105kDa
Enables importin-alpha family protein binding activity and protein kinase binding activity. Involved in activation of protein kinase activity; microtubule cytoskeleton organization; and negative regulation of microtubule depolymerization. Located in intercellular bridge; mitotic spindle; and nucleoplasm. Colocalizes with spindle pole.
Purification Method:
Affinity purification
Gene ID:
22974
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from A-549 cells, using TPX2 Rabbit pAb (CAB18327) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
