The TPSB2 Polyclonal Antibody (CAB15887) is a valuable tool for researchers studying TPSB2, a protease released by activated mast cells that plays a role in various physiological and pathological processes. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the TPSB2 protein, this antibody enables precise detection and analysis in different cell types, making it an ideal choice for studies in immunology, allergy, and inflammatory diseases.
TPSB2, also known as tryptase beta 2, is involved in the activation of protease-activated receptor 2 (PAR2), which has been linked to inflammatory responses and tissue remodeling. Understanding the function of TPSB2 is crucial for unraveling its role in diseases such as asthma, allergic reactions, and fibrotic disorders. By using the TPSB2 Polyclonal Antibody, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.
Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human Mast Cell Tryptase (Mast Cell Tryptase (TPSB2)) (NP_077078.5).
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
Purification Method:
Affinity purification
Gene ID:
64499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Mast Cell Tryptase (TPSB2) Rabbit pAb (CAB15887) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
