The TPSAB1 Polyclonal Antibody (CAB2022) is a powerful tool for researchers studying TPSAB1, a gene encoding tryptase alpha/beta 1. Tryptase is a protease that plays a key role in allergic reactions and inflammatory responses. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, and is suitable for use in Western blot applications.By targeting the TPSAB1 protein, this antibody allows for accurate detection and analysis of tryptase alpha/beta 1 in a variety of cell types.
Its reliability and precision make it an invaluable asset for investigations in immunology, allergy research, and inflammation studies. Understanding the function and regulation of TPSAB1 can provide insights into the mechanisms underlying allergic diseases, inflammatory disorders, and potential therapeutic targets for intervention.
Product Name:
TPSAB1 Rabbit Polyclonal Antibody
SKU:
CAB2022
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 31-275 of human TPSAB1 (NP_003285.2).
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
Purification Method:
Affinity purification
Gene ID:
7177
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat skin, using TPSAB1 Rabbit pAb (CAB2022) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
