The TPMT Polyclonal Antibody (CAB1017) is a valuable tool for researchers studying the thiopurine S-methyltransferase (TPMT) enzyme, which plays a critical role in the metabolism of thiopurine drugs used in the treatment of autoimmune diseases and leukemia. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.TPMT is an important enzyme in the thiopurine drug pathway, responsible for inactivating and clearing these medications from the body. Variations in TPMT activity can impact drug efficacy and toxicity, making TPMT genotyping and phenotyping essential for personalized medicine approaches.
The TPMT Polyclonal Antibody binds specifically to TPMT protein, enabling precise detection and quantification in various cell types and tissues, making it an invaluable tool for pharmacogenomic studies and clinical research.By utilizing the TPMT Polyclonal Antibody, researchers can gain insights into TPMT expression levels, distribution, and functionality, facilitating the development of more tailored and effective treatment strategies for patients receiving thiopurine drugs. This antibody is a crucial resource for advancing our understanding of TPMT biology and its role in drug metabolism and therapeutic outcomes.
Product Name:
TPMT Rabbit Polyclonal Antibody
SKU:
CAB1017
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPMT (NP_000358.1).
This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X.
Purification Method:
Affinity purification
Gene ID:
7172
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from K562 cells, using TPMT Rabbit pAb (CAB1017) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
