The TPK1 Polyclonal Antibody (CAB4634) is a valuable tool for researchers studying TPK1, a key enzyme involved in thiamine biosynthesis and essential for cellular growth and development. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the TPK1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it well-suited for studies in biochemistry and metabolism research.TPK1 is a crucial enzyme in the thiamine pyrophosphate synthesis pathway, playing a vital role in cellular energy production and metabolism.
Dysregulation of TPK1 has been linked to various metabolic disorders and neurodegenerative diseases, making it a key target for further investigation in the field of molecular biology. By understanding the function and regulation of TPK1, researchers can gain valuable insights into cellular metabolism and potentially identify new therapeutic targets for metabolic disorders and neurological conditions.
Product Name:
TPK1 Rabbit Polyclonal Antibody
SKU:
CAB4634
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-243 of human TPK1 (NP_071890.2).
The protein encoded by this gene functions as a homodimer and catalyzes the conversion of thiamine to thiamine pyrophosphate, a cofactor for some enzymes of the glycolytic and energy production pathways. Defects in this gene are a cause of thiamine metabolism dysfunction syndrome-5.
Purification Method:
Affinity purification
Gene ID:
27010
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TPK1 antibody (CAB4634) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
