The Tryptophan Hydroxylase 1 (TPH1) Polyclonal Antibody (PAC046294) offered by Assay Genie is specifically designed for research involving TPH1, an enzyme involved in the biosynthesis of serotonin. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the TPH1 protein, this antibody enables detection and analysis in various cell types, making it a valuable tool for studies in neuroscience, psychology, and mental health research.TPH1 is a key enzyme in the regulation of serotonin levels in the brain and peripheral tissues, playing a crucial role in mood, behavior, and emotional regulation.
Dysregulation of TPH1 activity has been implicated in various psychiatric disorders, including depression, anxiety, and schizophrenia. Research into the function of TPH1 and its role in serotonin biosynthesis is essential for developing novel treatments and therapies for these conditions.With its high reactivity and validation for Western blot applications, the TPH1 Polyclonal Antibody (PAC046294) from Assay Genie is a reliable tool for researchers seeking to explore the role of TPH1 in serotonin biosynthesis and its implications for mental health and neurological disorders.
Antibody Name:
Tph1 Antibody (PACO46294)
Antibody SKU:
PACO46294
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Mouse
Immunogen:
Recombinant Mouse Tryptophan 5-hydroxylase 1 protein (1-447AA)
TPH1: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described. Protein type: Amino Acid Metabolism - tryptophan; EC 1.14.16.4; OxidoreductaseCellular Component: cytoplasm; neuron projectionMolecular Function: amino acid binding; iron ion binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen; tryptophan 5-monooxygenase activityBiological Process: aromatic amino acid family metabolic process; bone remodeling; metabolic process; negative regulation of ossification; positive regulation of fat cell differentiation; serotonin biosynthetic process
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase family. The encoded protein is one of two tryptophan hydroxylase enzymes that catalyze the first and rate limiting step in the biosynthesis of the hormone and neurotransmitter, serotonin. This gene is expressed in peripheral organs, while tryptophan hydroxylase 2 is expressed in neurons. The encoded protein is involved in the development of hypoxia-induced elevations in pulmonary pressures and pulmonary vascular remodeling, and has also been implicated as a regulator of immune tolerance. Disruption of this gene is associated with cardiac dysfunction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]
