The TP53BP1 Polyclonal Antibody is a powerful tool for researchers studying TP53BP1, a protein involved in DNA damage response and repair processes. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it a valuable tool for investigating TP53BP1 expression and function in various cell types and tissues.TP53BP1, also known as tumor protein p53 binding protein 1, plays a critical role in maintaining genomic stability and cell cycle regulation. Dysregulation of TP53BP1 has been associated with various human diseases, including cancer and neurodevelopmental disorders.
By targeting TP53BP1, researchers can gain insights into its role in DNA damage repair pathways and its potential as a therapeutic target for cancer treatment.The TP53BP1 Polyclonal Antibody has been validated for use in Western blot applications, enabling researchers to detect and analyze TP53BP1 protein levels in experimental samples. Its high specificity and sensitivity make it a reliable tool for studies in cell biology, oncology, and DNA repair mechanisms. By utilizing this antibody, researchers can further our understanding of TP53BP1 function and its implications in disease pathology.
Product Name:
TP53BP1 Polyclonal Antibody
SKU:
CAB21632
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 895-1031 of human TP53BP1 (NP_005648.1).
Sequence:
FCES SSET PFHF TLPK EGDI IPPL TGAT PPLI GHLK LEPK RHST PIGI SNYP ESTI ATSD VMSE SMVE THDP ILGS GKGD SGAA PDVD DKLC LRMK LVSP ETEA SEES LQFN LEKP ATGE RKNG STAV AESV ASPQ K
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
p202; 53BP1; TDRD30; p53BP1; TP53BP1
Positive Sample:
Mouse brain,Rat uterus
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus, centromere, kinetochore.
Calculated MW:
214kDa
Observed MW:
300-450kDa
This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
7158
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TP53BP1 Rabbit pAb (CAB21632) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
