The TP53 Polyclonal Antibody (CAB5761) is a valuable tool for researchers studying TP53, a tumor suppressor gene commonly mutated in various cancers. This antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the TP53 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for investigations in oncology and cancer biology.TP53, also known as p53, is a key player in regulating cell cycle progression and apoptosis, serving as a guardian of the genome by preventing the growth of abnormal cells that could lead to cancer development.
Mutations in TP53 have been associated with a wide range of malignancies, highlighting the importance of understanding its role in tumor suppression and DNA damage response. Research on TP53 using this antibody can provide insights into cancer progression, treatment resistance, and potential therapeutic strategies targeting this pathway.
Product Name:
[KO Validated] p53 Rabbit Polyclonal Antibody
SKU:
CAB5761
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 218-390 of human p53 (NP_035770.2).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Immunoprecipitation analysis of 200 μg extracts of U-251MG cells using 3 μg p53 antibody (CAB5761). Western blot was performed from the immunoprecipitate using p53 antibody (CAB5761) at a dilution of 1:1000.
