The TP53 Polyclonal Antibody (CAB3185) is a valuable tool for researchers studying the TP53 gene, known as the "guardian of the genome" for its role in preventing cancer development. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.TP53 is a crucial tumor suppressor gene that regulates cell growth and apoptosis in response to DNA damage. Mutations in TP53 are commonly found in various types of cancer, making it an important target for cancer research.
The TP53 Polyclonal Antibody detects the TP53 protein, allowing researchers to study its expression and function in different cell types and disease states.By using the TP53 Polyclonal Antibody, researchers can gain insights into the mechanisms underlying cancer development and potentially identify new therapeutic targets for cancer treatment. Its high reactivity and specificity make it an essential tool for studies aiming to understand the role of TP53 in tumorigenesis and tumor progression.
Product Name:
p53 Rabbit Polyclonal Antibody
SKU:
CAB3185
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-393 of human p53 (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using p53 Rabbit pAb (CAB3185) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
