The TP53 Polyclonal Antibody (CAB11232) is a valuable tool for researchers studying the tumor protein p53 (TP53), a critical tumor suppressor gene involved in regulating cell growth and preventing cancer development. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been rigorously validated for use in Western blot applications.TP53, also known as the "guardian of the genome," is a key player in maintaining genomic stability and preventing the proliferation of damaged cells that could lead to cancer. Mutations in the TP53 gene are commonly found in various types of cancer, highlighting its importance in tumor suppression and cell cycle control.
By targeting the TP53 protein, researchers can gain insight into the mechanisms underlying cancer development and potentially identify new therapeutic strategies for treating cancer.The TP53 Polyclonal Antibody enables accurate detection and analysis of TP53 protein expression in a range of cell types, making it an invaluable tool for cancer research and drug development. By studying the role of TP53 in cell cycle regulation and DNA repair processes, researchers can further our understanding of cancer biology and potentially identify new targets for anti-cancer therapies.
Product Name:
[KO Validated] p53 Rabbit Polyclonal Antibody
SKU:
CAB11232
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 95-390 of mouse p53 (NP_035770.2).
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
22059
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Rat brain using p53 Rabbit pAb (CAB11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
