The TP53 Polyclonal Antibody (CAB0263) is an essential tool for researchers studying the tumor protein p53 (TP53), also known as the "guardian of the genome." TP53 is a critical tumor suppressor gene that plays a key role in regulating cell growth, apoptosis, and DNA repair, making it a key focus in cancer research.Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for Western blot applications. It binds specifically to the TP53 protein, allowing for precise detection and analysis in various cell types. Researchers in the fields of oncology, molecular biology, and genetics will find this antibody invaluable for their studies on TP53 and its role in cancer development and progression.
By delving into the functions and dysregulation of TP53, researchers can gain insights into the mechanisms underlying cancer formation and potentially identify novel therapeutic targets for the treatment of various malignancies. The TP53 Polyclonal Antibody (CAB0263) is a valuable tool for advancing our understanding of cancer biology and developing new strategies for cancer diagnosis and treatment.
Product Name:
p53 Rabbit Polyclonal Antibody
SKU:
CAB0263
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53 (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells using p53 Rabbit pAb(CAB0263) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
