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Total Sulfhydryl Group/Total Thiol (-SH) Assay Kit - Colorimetric (MAES0167)

SKU:
MAES0167
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Animal Tissue
Sensitivity:
9.91 umol/L
Range:
9.91-1000 umol/L
Assay Time:
20 min
Shipping:
Gel Pack
Research Area:
Oxidative Stress
Antioxidant
€299
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Total Sulfhydryl Group/Total Thiol (-SH) Assay Kit - Colorimetric
Product Code:MAES0167
Product Size:96 Assays
Sample Type:Serum, plasma, animal tissue
Assay Time:20 min
Instrument:Microplate reader
Sensitivity:9.91 µmol/L
Detection Range:9.91-1000 µmol/L
Recovery Rate:104
Inter CV:2.9
Inter CV:2.5

Total sulfhydryl group/total thiol (-SH) is an important protein structure and REDOX reactive group in organisms. Sulfhydryl is one of the most active and ubiquitous ligands in biological systems. It is found in most proteins, but also in some low molecular weight substances. Sulfhydryl groups play an important role in biochemical processes, not only for the REDOX mechanism, but also for the enhancement of the function of some hydrolyzed biocatalysts. Compounds containing sulfhydryl groups are called thiols. Decreased thiol levels are found in various diseases.

Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly.

Shelf Life:6 Months
Storage:2-8°C
Shipping:Gel Pack
Research Area:Oxidative stress

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