Total Protein Assay Kit (BA0174)

SKU:

BA0174

Product Type:

Assay

Instrument:

Microplate Reader

Sample Type:

Biological Samples

Research Area:

Amino Acid and Protein

Description

Description

Total Protein Assay Kit - Information

Assay Genie's fluorometric protein assay kit is based on an improved o-phthalaldehyde method. This reagent reacts with primary amines in protein or peptide and forms a blue fluorescent product, allowing detection of nanograms of proteins. The fluorescence intensity (Ex/Em = 360/450nm) is proportional to the protein concentration in the sample.

Applications

For quantitative determination of total protein

Total Protein Assay Kit -Key Features

Fast and sensitive. Assay is completed within a few minutes. Linear detection range of 0.05 - 200 ug/mL BSA.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Total Protein Assay Kit - Data Sheet
Kit Includes	Reagent: 20 mL Standard: 1 mL 1mg/mL BSA
Kit Requires	Pipetting devices, centrifuge tubes, black flat bottom 96-well plates and plate reader
Method of Detection	FL360/450nm
Detection Limit	50 ng/mL
Samples	Biological
Species	All
Protocol Length	10 min
Size	200 tests
Storage	For long-term storage, keep kit at -20°C
Shelf Life	6 months

More Details

The protein or peptide is known as the "building blocks of life" and is one of the most important macromolecules in life science. Protein determination is a very common practice. Simple, direct and automation-ready procedures for measuring protein or peptide concentration are very desirable.