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Total Cholesterol Assay Kit - Colorimetric (MAES0093)

SKU:
MAES0093
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Tissue, Cells, Cell Culture Supernatant
Sensitivity:
0.29 mmol/L
Range:
0.29-25.85 mmol/L
Assay Time:
20 min
Shipping:
Gel Pack
Research Area:
Diabetes & Obesity
€299
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Total Cholesterol Assay Kit - Colorimetric
Product Code:MAES0093
Product Size:96 Assays
Sample Type:Serum, plasma, tissue, cells, cell culture supernatant
Assay Time:20 min
Instrument:Microplate reader
Sensitivity:0.29 mmol/L
Detection Range:0.29-25.85 mmol/L
Recovery Rate:103
Inter CV:8.3
Inter CV:3.1

Cholesterol is a kind of sterol and lipid in cell membrane. Most of cholesterol in blood exists in the form of cholesterol ester. Lecithin-cholesterol acyltransferase in human plasma is an enzyme that catalyzes the formation of cholesterol ester. Cholesterol synthesized or deposited in peripheral cells returns to the liver through the reverse cholesterol transport system for reusing or regaining bile acids.

Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce â–³4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone.The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated.

Shelf Life:6 Months
Storage:2-8°C
Shipping:Gel Pack
Research Area:Lipids metabolism

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