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Total Cholesterol and Cholesteryl Ester Assay Kit - Fluorometric (MAES0007)

SKU:
MAES0007
Product Type:
Assay
Detection Method:
Fluorometric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Animal Tissue, Cells
Sensitivity:
0.12 umol/L
Range:
0.12-30 umol/L
Assay Time:
20 min
Shipping:
Gel Pack
Research Area:
Ferroptosis
Diabetes & Obesity
$659
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Total Cholesterol and Cholesteryl Ester Assay Kit - Fluorometric
Product Code:MAES0007
Product Size:96 Assays
Sample Type:Serum, plasma, animal tissue, cells
Assay Time:20 min
Instrument:Fluorescence Microplate Reader
Sensitivity:0.12 µmol/L
Detection Range:0.12-30 µmol/L
Recovery Rate:96
Inter CV:7.3
Inter CV:1.7

Cholesterol is a kind of sterol and lipid in cell membrane. Most of cholesterol in blood exists in the form of cholesterol ester. Lecithin-cholesterol acyltransferase in human plasma is an enzyme that catalyzes the formation of cholesterol ester. Cholesterol synthesized or deposited in peripheral cells returns to the liver through the reverse cholesterol transport system for reusing or regaining bile acids.

Total cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce â–³4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.

Shelf Life:6 Months
Storage:-20°C
Shipping:Gel Pack
Research Area:Lipids metabolism

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