DNA topoisomerase II alpha (TOP2A) Rabbit Monoclonal Antibody (CAB4389)
The Topoisomerase II Alpha Rabbit Monoclonal Antibody (CAB4389) is a valuable tool for researchers studying the role of the topoisomerase II alpha enzyme in DNA replication and transcription. This antibody, generated in rabbits, is highly specific and sensitive for detecting the topoisomerase II alpha protein in human samples. Validated for use in Western blot and immunohistochemistry applications, this antibody enables accurate detection and localization of topoisomerase II alpha in various cell types.Topoisomerase II alpha is an essential enzyme involved in DNA metabolism, playing a crucial role in unwinding and winding DNA strands during processes such as replication and transcription.
Dysregulation of this enzyme has been implicated in various diseases, including cancer, making it a promising target for therapeutic interventions. Understanding the expression and activity of topoisomerase II alpha is essential for advancing research in fields such as oncology and molecular biology.Overall, the Topoisomerase II Alpha Rabbit Monoclonal Antibody (CAB4389) offers researchers a reliable tool for investigating the functions and mechanisms of topoisomerase II alpha in cellular processes and disease pathology. Its high specificity and sensitivity make it suitable for a wide range of experimental applications, making it a valuable asset in scientific studies.
Product Name:
DNA topoisomerase II alpha (TOP2A) Rabbit Monoclonal Antibody
SKU:
CAB4389
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1432-1531 of human DNA topoisomerase II alpha (TOP2A) (P11388).
TOP2; TP2A; TOPIIA; TOP2alpha; DNA topoisomerase II alpha (TOP2A)
Positive Sample:
HeLa,Mouse testis
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, nucleoplasm.
Calculated MW:
174kDa/178kDa/179kDa/183kDa
Observed MW:
190kDa
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
Purification Method:
Affinity purification
Gene ID:
7153
Clone Number:
ARC0994
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DNA DNA topoisomerase II alpha (TOP2A) Rabbit mAb (CAB4389) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
