The Topo II Polyclonal Antibody is a powerful tool for researchers studying the Topoisomerase II enzyme, essential for DNA replication and repair. This antibody, generated in rabbits, has high specificity for human samples and is validated for use in Western blotting. By binding to the Topo II protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an invaluable asset for research in molecular biology and cancer studies.Topoisomerase II is a crucial enzyme involved in DNA metabolism, playing a key role in processes like chromosome condensation and segregation.
Dysregulation of Topo II has been linked to various diseases, including cancer, making it a promising target for therapeutic intervention. By studying the activity and expression of Topo II using this antibody, researchers can gain valuable insights into the mechanisms underlying these conditions, potentially leading to the development of novel treatments.
Product Name:
Topo IIα Polyclonal Antibody
SKU:
CAB24496
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1431-1531 of human Topo IIα (NP_001058.2).
TOP2A; TOP2; TP2A; DNA topoisomerase 2-alpha; Topo IIα
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, nucleoplasm.
Calculated MW:
174kDa/177kDa/178kDa/182kDa
Observed MW:
190kDa
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
Purification Method:
Affinity purification
Gene ID:
7153
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Topo IIα Rabbit pAb (CAB24496) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
