The TopBP1 Polyclonal Antibody (CAB12574) is a valuable tool for researchers studying Topoisomerase-Binding Protein 1 (TopBP1), a key protein involved in DNA replication and repair processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.TopBP1 is essential for maintaining genomic stability and preventing the accumulation of DNA damage, making it a crucial factor in cancer development and progression. By detecting and analyzing TopBP1 levels in various cell types, researchers can gain insight into its function and potential implications in cancer biology.
Furthermore, TopBP1 has been implicated in regulating cell cycle progression and DNA damage response pathways, making it a promising target for therapeutic interventions in cancer treatment. Understanding the role of TopBP1 in cellular processes is essential for developing targeted therapies that exploit its function for the benefit of patients with cancer and other diseases characterized by genomic instability.
Product Name:
TOPBP1 Rabbit Polyclonal Antibody
SKU:
CAB12574
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOPBP1 (NP_008958.2).
Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
171kDa
Observed MW:
171kDa
This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
Purification Method:
Affinity purification
Gene ID:
11073
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using TOPBP1 Rabbit pAb (CAB12574) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
