The TOP3B Polyclonal Antibody (CAB8469) is a valuable tool for researchers studying the TOP3B protein, which is involved in DNA repair and genome stability. This antibody, produced in rabbits, is highly specific to human samples and is ideal for use in Western blot applications. By binding to TOP3B, this antibody enables accurate detection and analysis of the protein in various cell types, making it essential for studies in genetics, cancer research, and neurobiology.TOP3B is crucial for resolving DNA topological stress and maintaining genomic integrity, highlighting its importance in processes such as DNA replication and transcription.
Mutations in TOP3B have been linked to various genetic disorders and cancer, underscoring the need for further research into its function and regulation. The TOP3B Polyclonal Antibody offers a reliable tool for investigating the role of TOP3B in these contexts, ultimately advancing our understanding of DNA repair mechanisms and disease mechanisms.
Product Name:
TOP3B Rabbit Polyclonal Antibody
SKU:
CAB8469
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TOP3B (NP_003926.1).
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Purification Method:
Affinity purification
Gene ID:
8940
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TOP3B antibody (CAB8469) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
