The TOP3A Polyclonal Antibody (CAB16987) is a valuable tool for researchers studying TOP3A, a protein involved in DNA repair and regulation of gene expression. This rabbit-derived antibody is highly specific to human samples and is validated for use in Western blot applications. By binding to the TOP3A protein, this antibody allows for the accurate detection and analysis of TOP3A in various cell types, making it an ideal choice for studies in molecular biology and cancer research.TOP3A, a topoisomerase enzyme, plays a crucial role in maintaining genome stability and ensuring proper DNA replication.
Dysregulation of TOP3A has been implicated in various diseases, including cancer and genetic disorders. By studying the function of TOP3A, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the TOP3A Polyclonal Antibody (CAB16987) offers researchers a reliable tool for investigating the role of TOP3A in health and disease. Its high specificity and sensitivity make it a valuable asset for studies seeking to unravel the complex functions of this important protein.
Product Name:
TOP3A Rabbit Polyclonal Antibody
SKU:
CAB16987
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP3A (XP_011522303.1).
mitochondrial matrix, nucleoplasm, nucleus, PML body
Calculated MW:
112kDa
Observed MW:
112kDa
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
7156
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse testis, using TOP3A Rabbit pAb (CAB16987) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
