DNA topoisomerase II alpha (TOP2A) Rabbit Polyclonal Antibody (CAB16440)
The TOP2A Polyclonal Antibody (CAB16440) is a valuable tool for researchers studying the TOP2A protein, which plays a key role in DNA replication and repair processes. This antibody is raised in rabbits and has been validated for use in Western blot applications, providing reliable detection and analysis of the TOP2A protein in a variety of cell types.TOP2A is essential for DNA unwinding during replication and is a target for a number of anti-cancer drugs.
Research into the function and regulation of TOP2A is crucial for understanding its role in cancer development and identifying potential therapeutic strategies. The TOP2A Polyclonal Antibody enables researchers to investigate the expression and activity of this important protein, making it an invaluable tool for studies in oncology and molecular biology.
Product Name:
DNA topoisomerase II alpha (TOP2A) Rabbit Polyclonal Antibody
SKU:
CAB16440
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human DNA topoisomerase II alpha (TOP2A) (NP_001058.2).
Sequence:
EEEN EESD NEKE TEKS DSVT DSGP TFNY LLDM PLWY LTKE KKDE LCRL RNEK EQEL DTLK RKSP SDLW KEDL ATFI EELE AVEA KEKQ DEQV GLPG KGGK A
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
TOP2; TP2A; TOPIIA; TOP2alpha; DNA topoisomerase II alpha (TOP2A)
Positive Sample:
HeLa,293T
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, nucleoplasm.
Calculated MW:
174kDa
Observed MW:
190kDa
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
Purification Method:
Affinity purification
Gene ID:
7153
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DNA topoisomerase II alpha (TOP2A) antibody (CAB16440) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
